Manija Kazmi Manija Kazmi, Research Specialist & Laboratory Manager
Laboratory of Molecular Biology and Biochemistry
Rockefeller Research Building 510

The Rockefeller University, Box 187
1230 York Avenue
New York, NY 10065

Telephone: 212-327-8284
Fax: 212-327-7904

kazmim@mail.rockefeller.edu


pdfBiosketch

Education:
M.S. in Molecular Biology, New York University 1997
B.S. in Biology, Queens College 1992

 

Research:
My role in the lab is now primarily lab manager. I mainly assist other lab members with their ongoing projects. My most recent research has been to elucidate the function of pepducins, which are novel cell-penetrating peptides that act as inhibitors of signalling from receptors to G proteins.



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Awards:

Published Papers:

15).
Ye S., Köhrer C., Huber T., Kazmi M., Sachdev P., Yan E.C., Bhagat A., RajBhandary U.L., Sakmar T.P.
Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis.
J Biol Chem 283: 1525-33 (2008)

PMID: 17993461 [PubMed - indexed for MEDLINE]

14).
Lewis, J.W., Szundi, I., Kazmi, M.A., Sakmar, T.P., & Kliger, D. S. 
Proton movement and photointermediate kinetics in rhodopsin mutants
Biochemistry 45: 5430-9 (2006)

PMID: 16634624 [PubMed - indexed for MEDLINE]
13).
Su, C.Y., Luo, D.G., Terakita, A., Shichida, Y., Liao, H.W., Kazmi, M.A., Sakmar, T.P., & Yau, K.W. 
Parietal-eye phottransduction components and their potential evolutionary implications
Science 311: 1617-21 (2006)

PMID: 16543463 [PubMed - indexed for MEDLINE]

12).
Lewis, J.W., Szundi, I., Kazmi, M.A., Sakmar, T.P., and Kliger, D.S. 
Time-resolved photointermediate changes in rhodopsin glutamic acid 181 mutants
Biochemistry 43: 12614-21 (2004)

PMID: 15449951 [PubMed - indexed for MEDLINE]
11).
Yan, E. C., Ganim, Z., Kazmi, M. A., Chang, B. S. W., Sakmar, T. P. & Mathies, RA
Resonance Raman Analysis of the Mechanism of Energy Storage and Chromophore Distortion in the Primary Visual Photoproduct
Biochemistry 43:10867-10876 (2004)

PMID: 15323547 [PubMed - indexed for MEDLINE]
10).
Yan, E. C., Kazmi, M. A., Ganim, Z., Hou, J. M., Pan, D., Chang, B. S. W., Sakmar, T. P. & Mathies, R.  A. 
Retinal Counterion Switch in the Photoactivation of the G Protein-Coupled Receptor Rhodopsin
Proc Natl Acad Sci U S A  100:9262-9267 (2003)

PMID: 12835420 [PubMed - indexed for MEDLINE]
9).
Chang, B.S.W., Jonsson K., Kazmi, M.A., Donoghue, M.J., and Sakmar, T.P. 
Recreating a functional ancestral archosaur visual pigment
Mol. Biol. Evol. 19: 1483-9 (2002)

PMID: 12200476 [PubMed - indexed for MEDLINE]
8).
Yan, E. C., Kazmi, M.A., De S., Chang, B. S. W., Seibert, C. Marin E. P., Mathies, R.A., and Sakmar, T.P. 
Function of extracellular loop 2 in rhodopsin: glutamic acid 181 modulates stability and absorption wavelength of metarhodopsin II
Biochemistry  19:3620-7 (2002)

PMID: 11888278 [PubMed - indexed for MEDLINE]
7).
Chang, B.S.W., Manija A. Kazmi and Thomas P. Sakmar. 
Synthetic gene technology: Applications to ancestral gene reconstruction and structure-function studies of receptors
Methods in  Enzymology 343: 274-94 (2002)

PMID: 11665573 [PubMed - indexed for MEDLINE]
6).
Kazmi, M.A., L.A. Snyder, A.M. Cypess, S.G. Graber, T.P. Sakmar. 
Selective reconstitution of human D4 dopamine receptor variants with Gi alpha subtypes
Biochemistry 39:3734-44 (2000)

PMID: 10736173 [PubMed - indexed for MEDLINE]
5).
Ostrer, H., R.K. Pullarkat, and M.A. Kazmi. 
Glycosylation and palmitoylation are not required for the formation of the X-linked cone opsin visual pigments
Mol. Vis. 10;4:28 (1998)

PMID: 9852167 [PubMed - indexed for MEDLINE]
4).
Ostrer, H., and M.A. Kazmi. 
Mutation of a conserved proline disrupts the retinal-binding pocket of the X-linked cone opsins
Mol. Vis. 29; 3:16 (1997)

PMID: 9479007 [PubMed - indexed for MEDLINE]
3).
Kazmi, M.A., T.P. Sakmar, and H. Ostrer. 
Mutation of a conserved cysteine in the X-linked cone opsins causes color vision deficiencies by disrupting protein folding and stability
Invest. Ophthalmol. Vis. Sci. 38:1074-81 (1997)

PMID: 9152227 [PubMed - indexed for MEDLINE]
2).
Kazmi, M. A., Dubin, R. A., Oddoux, C., and Ostrer, H.
High-level expression of visual pigments in  transfected cells
Biotechniques 21:304-311 (1996)

PMID: 8862817 [PubMed - indexed for MEDLINE]

1).
Dubin R. A., Kazmi M. A., Ostrer H.
Inverted repeats are required for circularization of the mouse Sry  transcript
Gene 167:245-248 (1995)

PMID: 8566785 [PubMed - indexed for MEDLINE]

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Recent Presentations:


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Posters:
8).
Ye, S.; Huber, T., Sakmar, T.P.
Fluorescent Labeling of GPCRs by the Site Specific Incorporation of Keto and Azido Amino Acids.
Biophysical Society Meeting, Boston, US, March, 2009.
7).
Ye, S.; Huber, T., Sakmar, T.P.
Site-Specific Labeling of Heptahelical Receptors Containing Keto and Azido Amino Acids.
Keystone symposia, Cambridge, UK, September, 2008.
6).
Ye, S.; Huber, T., Sakmar, T.P.
Fluorescent Labeling of GPCRs by the Site Specific Incorporation of Unnatural Amino Acids.
Rockefeller University Postdoctoral Retreat, New York, September 2007 and 2008.
5).
Ye, S.; Strzalka, J.W., Discher, B.M.; Noy, D., Moser, C.C.; Dutton, P.L., Blasie, J.K.
Design and characterization of artificial integral membrane proteins for vectorial electron transfer across soft interfaces.
226th ACS National Meeting, New York, September, 2004.
4).
Ye, S.; Strzalka, J.W., Churbanova, I.Y., Johansson, J.S., Blasie, J.K.
Model Membrane Protein for Binding Volatile Anesthetics.
48th Biophysical Society Annual Meeting, Baltimore, February, 2004.
3).
Ye, S.; Discher, B.M., Strzalka, J.W., Moser, C.C., Dutton, P.L., Blasie, J.K.
Design and physical characterization of vectorially oriented minimal electron-transfer protein monolayer at the air/water interface.
ACS National Meeting, New York, September, 2003.
2).
Ye, S.; Discher, B.M., Strzalka, J.W., Moser, C.C., Dutton, P.L., Ocko, B.M., Blasie, J.K.
Design and Physical Characterization of Vectorially Oriented Heme-Binding Peptide Monolayer.
36th Middle Atlantic Regional Meeting of ACS, Princeton, June, 2003.

1).
Ye, S.; Discher, B.M., Chen, X.X., Moser, C.C., Dutton, P.L., Ocko, B.M., Blasie, J.K.
Maquette Peptide Design and Its Applications in Biomaterial Engineering.
Institute of Medicine and Engineering of the University of Pennsylvania Annual Meeting, Philadelphia, Pennsylvania, December, 2001.